Journal: iScience

Article Title: A pan-tissue multi-omics resource atlas of coordinated immune, metabolic, and fibrotic alterations in diet-induced MASH

doi: 10.1016/j.isci.2026.117139

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Article Snippet: Fatty Acid Synthase (FASN) Rabbit mAb , ABclonal , Cat# A19050; RRID: AB_2100796.

Techniques: Recombinant, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Activity Assay, Transcriptomics, Mass Spectrometry, Reverse Transcription Polymerase Chain Reaction, Software

Journal: Computational and Structural Biotechnology Journal

Article Title: Epigenomics-Guided Multi-Omics Integration Uncovers a Lipid-Metabolic Signature with Translational Utility in Bladder Cancer

doi: 10.34133/csbj.0139

Figure Lengend Snippet: Pathway enrichment analysis of the 25-gene signature. (A) Reactome enrichment analysis identified significant pathways, including fatty acid metabolism, cholesterol biosynthesis, and nuclear receptor signaling. (B) WikiPathways analysis corroborated the involvement of these genes in lipid metabolism pathways, including SREBF and MIR33 (microRNA 33) in cholesterol and lipid homeostasis. (C) Gene–pathway interaction network highlighting the central roles of lipid metabolism regulators fatty acid (FA) synthase ( FASN ) and stearoyl-coenzyme A (CoA) desaturase ( SCD ) and other genes involved in the cell cycle and membrane trafficking, including TPD52 like 1 ( TPD52L1 ), leucine-rich repeats and immunoglobulin-like domains 1 ( LRIG1 ), keratin 7 ( KRT7 ), homeobox C4 ( HOXC4 ), and CDC42 effector protein 3 ( CDC42EP3 ).

Article Snippet: In summary, 5,637 cells (1,000 cells per well) and J82 cells (500 cells per well) were seeded into 6-well plates and incubated at 37 °C for 24 h. The following day, one group served as the control and was maintained in the growth medium, while the experimental group received an addition of 50 μM FASN inhibitor (MedChemExpress, TVB-3664, catalog no. HY-120062) and SCD inhibitor (MedChemExpress, A939572, catalog no. HY-50709) to the growth medium and was cultured for a duration of 13 d. Subsequently, the cells were fixed with 4% formaldehyde (Sigma-Aldrich) and stained with 0.5% crystal violet (Sigma-Aldrich).

Techniques: Membrane

Journal: Computational and Structural Biotechnology Journal

Article Title: Epigenomics-Guided Multi-Omics Integration Uncovers a Lipid-Metabolic Signature with Translational Utility in Bladder Cancer

doi: 10.34133/csbj.0139

Figure Lengend Snippet: Prognostic validation and functional assessment of lipid metabolism regulators, fatty acid synthase ( FASN ) and stearoyl-coenzyme A desaturase ( SCD ). (A) Kaplan–Meier survival analysis indicating the prognostic significance of high expression levels of FASN (hazard ratio [HR] = 1.41) and SCD (HR = 1.8) in patients with bladder cancer (BLCA). (B) Expression analysis across stages revealed no significant variation (ANOVA, P = 0.3). (C) Colony formation assays demonstrating significantly reduced proliferation in BLCA cell lines (J82 and 5637) treated with the FASN inhibitor (FASNi; TVB-3664) and SCD inhibitor (SCDi; A939572). (D) Migration assays confirmed significantly impaired motility upon FASN and SCD inhibition, underscoring their roles in tumor aggressiveness. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Article Snippet: In summary, 5,637 cells (1,000 cells per well) and J82 cells (500 cells per well) were seeded into 6-well plates and incubated at 37 °C for 24 h. The following day, one group served as the control and was maintained in the growth medium, while the experimental group received an addition of 50 μM FASN inhibitor (MedChemExpress, TVB-3664, catalog no. HY-120062) and SCD inhibitor (MedChemExpress, A939572, catalog no. HY-50709) to the growth medium and was cultured for a duration of 13 d. Subsequently, the cells were fixed with 4% formaldehyde (Sigma-Aldrich) and stained with 0.5% crystal violet (Sigma-Aldrich).

Techniques: Biomarker Discovery, Functional Assay, Expressing, Migration, Inhibition